feng zhang (Addgene inc)
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feng zhang
Feng Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/feng+zhang/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm41920822-295-17-20
Average 96 stars, based on 2935 article reviews
Feng Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/feng+zhang/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm41920822-295-17-20
Average 96 stars, based on 2935 article reviews
feng zhang - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Structures and molecular mechanisms of RAD54B in modulating homologous recombination Article Snippet: .. Allele 2 showed an insertion of 171 bp at aa582, resulting in a premature stop codon after 15 of the inserted basepairs. pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Article Title: Mitotic BLM functions are required to maintain genomic stability Article Snippet: .. The Cas9/sgRNA plasmid was constructed using the px330-U6-Chimeric_BB-CBh_hSPCas9 plasmid obtained from Article Title: CBP-IDRs regulate acetylation and gene expression Article Snippet: .. PX458 was a gift from Article Title: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting Article Snippet: Fluorescence-activated cell sorting (FACS) was performed using the FACSAria Fusion flow cytometer (BD Biosciences) to isolate GFP-positive and 7-AAD-negative cells. .. Genomic DNA was subsequently extracted using the NaOH lysis method. pSpCas9(BB)-2A-GFP (PX458) was a gift from Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation. Article Snippet: .. A guide RNA sequence targeting the C-terminal region of ADNP was inserted into PX459, a gift from Article Title: A low-toxicity circular single-stranded DNA platform for safe and potent IL-12 immunotherapy against hepatocellular carcinoma. Article Snippet: .. The pT3-EF1a-Myc vector was a kind gift of Dr. Xin Chen, UCSF San Francisco, PT2/C-Luc//PGK-SB13 was a gift from John Ohlfest (Addgene plasmid # 20207) and pX330-U6-Chimeric_BB-CBhhSpCas9 was a gift from Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Transformants were picked and plasmid DNA was purified with the QIAprep Spin Miniprep Kit (QIAGEN, Catalog no: 27106) and/or the NucleoBond Xtra Midi kit (MACHEREY-NAGEL, Catalog no: 740410.50). .. The CRISPR/Cas9-mediated insertions of the ANCH3 sequence were performed using SpCas9 and the SpCas9 HF1 variant (N497A; R661A; Q695A; Q926A). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift by Article Title: An upstream open reading frame represses translation of the neuronal potassium channel KCNQ2 Article Snippet: .. A vector for expressing sgRNA (pSpCas9(BB)-2A-GFP (PX458)) was obtained as a generous gift from Construct:Article Title: Mitotic BLM functions are required to maintain genomic stability Article Snippet: .. The Cas9/sgRNA plasmid was constructed using the px330-U6-Chimeric_BB-CBh_hSPCas9 plasmid obtained from Lysis:Article Title: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting Article Snippet: Fluorescence-activated cell sorting (FACS) was performed using the FACSAria Fusion flow cytometer (BD Biosciences) to isolate GFP-positive and 7-AAD-negative cells. .. Genomic DNA was subsequently extracted using the NaOH lysis method. pSpCas9(BB)-2A-GFP (PX458) was a gift from Sequencing:Article Title: ADNP regulates chromatin architecture and lineage fidelity during neural differentiation. Article Snippet: .. A guide RNA sequence targeting the C-terminal region of ADNP was inserted into PX459, a gift from Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Transformants were picked and plasmid DNA was purified with the QIAprep Spin Miniprep Kit (QIAGEN, Catalog no: 27106) and/or the NucleoBond Xtra Midi kit (MACHEREY-NAGEL, Catalog no: 740410.50). .. The CRISPR/Cas9-mediated insertions of the ANCH3 sequence were performed using SpCas9 and the SpCas9 HF1 variant (N497A; R661A; Q695A; Q926A). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift by CRISPR:Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Transformants were picked and plasmid DNA was purified with the QIAprep Spin Miniprep Kit (QIAGEN, Catalog no: 27106) and/or the NucleoBond Xtra Midi kit (MACHEREY-NAGEL, Catalog no: 740410.50). .. The CRISPR/Cas9-mediated insertions of the ANCH3 sequence were performed using SpCas9 and the SpCas9 HF1 variant (N497A; R661A; Q695A; Q926A). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift by Variant Assay:Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Transformants were picked and plasmid DNA was purified with the QIAprep Spin Miniprep Kit (QIAGEN, Catalog no: 27106) and/or the NucleoBond Xtra Midi kit (MACHEREY-NAGEL, Catalog no: 740410.50). .. The CRISPR/Cas9-mediated insertions of the ANCH3 sequence were performed using SpCas9 and the SpCas9 HF1 variant (N497A; R661A; Q695A; Q926A). pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift by Expressing:Article Title: An upstream open reading frame represses translation of the neuronal potassium channel KCNQ2 Article Snippet: .. A vector for expressing sgRNA (pSpCas9(BB)-2A-GFP (PX458)) was obtained as a generous gift from |